FastQCFastQC Report
Sat 23 Sep 2023
SRR13269806.TAZ.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13269806.TAZ.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44410668
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCGAACATCTCGT20414924.5968504684505085Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG9856782.2194622247069105Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCGAACATCGCGT1543030.3474457983833974Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGC1332600.30006303890767866Illumina Paired End Adapter 2 (96% over 28bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCGAACAGCTCGT510010.11483952459350533Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCGAACATGTCGT502090.11305616929698063Illumina Paired End PCR Primer 2 (97% over 39bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG448650.10102302446790487Illumina Paired End PCR Primer 2 (100% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)